Hotspot

ycf15-trnL-CAA

IRb · 94031-94785 · 755 bp

Back to Scolopia

Pi 0.00265

MarkerSeek score 60.30

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 755
variable_sites: 2
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	GCGAGCAACCAGAGCTATTA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.8	58.0	871 bp	1.0	78.3
ycf15-trnL-CAA_p2	GCGAGCAACCAGAGCTATTA	TGAAATGGTAGACACGCGAG	50.0	50.0	57.8	58.0	873 bp	1.0	78.3
ycf15-trnL-CAA_p3	GCGAGCAACCAGAGCTATTA	GCCTTGGTGGTGAAATGGTA	50.0	50.0	57.8	58.1	883 bp	1.0	77.7
ycf15-trnL-CAA_p4	GCGAGCAACCAGAGCTATTA	TGGTGAAATGGTAGACACGC	50.0	50.0	57.8	58.2	876 bp	1.0	76.8
ycf15-trnL-CAA_p5	GCGAGCAACCAGAGCTATTA	GAAATGGTAGACACGCGAGA	50.0	50.0	57.8	57.7	872 bp	1.0	76.2