Hotspot

petA-psbJ

LSC · 62683-63844 · 1162 bp

Back to Scolopia

Pi 0.00182

MarkerSeek score 56.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1162
variable_sites: 2
pi: 0.00182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.4	57.6	1210-1273 bp	1.0	68.5
petA-psbJ_p2	TGGCATCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.4	57.6	1211-1274 bp	1.0	68.5
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.2	57.6	1159-1222 bp	1.0	67.1
petA-psbJ_p4	TTGGCATCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	40.9	50.0	58.1	57.6	1212-1275 bp	1.0	64.6
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGAA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.9	57.6	1159-1222 bp	1.0	64.6