Hotspot

rpoC1-rpoB

LSC · 22784-22809 · 26 bp

Back to Scolopia

Pi 0.0385

MarkerSeek score 74.60

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GTTGAGGCGAAACTGATCCA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.2	58.0	174 bp	1.0	78.6
rpoC1-rpoB_p2	TGTTGAGGCGAAACTGATCC	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.2	58.0	175 bp	1.0	78.6
rpoC1-rpoB_p3	GTTGAGGCGAAACTGATCCA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.2	57.9	157 bp	1.0	78.2
rpoC1-rpoB_p4	TGTTGAGGCGAAACTGATCC	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.2	57.9	158 bp	1.0	78.2
rpoC1-rpoB_p5	GTTGAGGCGAAACTGATCCA	GGCTCTGGAACTGAAGCATT	50.0	50.0	58.2	58.2	148 bp	1.0	77.7