Hotspot

ycf1

SSC · 123576-129053 · 5478 bp

Back to Scleromitrion

Pi 0.0128

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 70
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCTACAGTTGTTCTTGGT	GCCCGTCTGTCTGTAAAAA	45.0	47.4	57.0	55.5	272 bp	1.0	45.6
ycf1_p2	ACAGTTGTTCTTGGTCCAACA	GCCCGTCTGTCTGTAAAAA	42.9	47.4	57.9	55.5	267 bp	1.0	45.1
ycf1_p3	TGCCTACAGTTGTTCTTGGTC	GCCCGTCTGTCTGTAAAAA	47.6	47.4	58.2	55.5	272 bp	1.0	44.5
ycf1_p4	TTGCCTACAGTTGTTCTTGGT	GCCCGTCTGTCTGTAAAAA	42.9	47.4	57.7	55.5	273 bp	1.0	43.4
ycf1_p5	CCTACAGTTGTTCTTGGTCCA	GCCCGTCTGTCTGTAAAAA	47.6	47.4	57.5	55.5	270 bp	1.0	42.1