Hotspot

rpl16

LSC · 80698-82201 · 1504 bp

Back to Scleromitrion

Pi 0.0168

MarkerSeek score 55.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1504
variable_sites: 25
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACGCTTTAGCTCTTTACGGG	GTTGGTTTGCACAACCCAAA	50.0	45.0	58.0	57.9	2558-2594 bp	1.0	80.8
rpl16_p2	CGCTTTAGCTCTTTACGGGT	GTTGGTTTGCACAACCCAAA	50.0	45.0	58.0	57.9	2557-2593 bp	1.0	80.8
rpl16_p3	ACGCTTTAGCTCTTTACGGG	AATCCAAATTGCGGGTCGTA	50.0	45.0	58.0	57.9	2137-2173 bp	1.0	80.8
rpl16_p4	CGCTTTAGCTCTTTACGGGT	AATCCAAATTGCGGGTCGTA	50.0	45.0	58.0	57.9	2136-2172 bp	1.0	80.8
rpl16_p5	GATCAATAACAACCGCAGCG	GTTGGTTTGCACAACCCAAA	50.0	45.0	57.9	57.9	2610-2646 bp	1.0	80.1