Hotspot

trnN-GUU-trnR-ACG

IRa · 129453-130029 · 577 bp

Back to Scleromitrion

Pi 0.0104

MarkerSeek score 62.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 577
variable_sites: 6
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	GCGAACAGAACAGATCCAGT	50.0	50.0	58.2	57.9	714 bp	1.0	78.7
trnN-GUU-trnR-ACG_p2	CGATTGGTCGTAGGTTCGAA	GCGAACAGAACAGATCCAGT	50.0	50.0	57.7	57.9	718 bp	1.0	77.8
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	670 bp	1.0	77.7
trnN-GUU-trnR-ACG_p4	GGTTCGAATCCTACTTGGGG	GCGAACAGAACAGATCCAGT	55.0	50.0	57.7	57.9	706 bp	1.0	77.4
trnN-GUU-trnR-ACG_p5	TCAGTAGCTCAGCGGTAGAG	GCGAACAGAACAGATCCAGT	55.0	50.0	58.3	57.9	753 bp	1.0	77.3