Hotspot

rpl22-rps19

LSC · 83411-83540 · 130 bp

Back to Scleromitrion

Pi 0.0396

MarkerSeek score 66.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 4
pi: 0.0396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GAACGTCCGCGAATTTGATC	AAATTTCCGAGGACACGCAA	50.0	45.0	57.9	58.4	248-277 bp	1.0	81.4
rpl22-rps19_p2	GAACGTCCGCGAATTTGATC	AATTTCCGAGGACACGCAAA	50.0	45.0	57.9	58.4	247-276 bp	1.0	81.4
rpl22-rps19_p3	GAACGTCCGCGAATTTGATC	ATTTCCGAGGACACGCAAAA	50.0	45.0	57.9	58.4	246-275 bp	1.0	81.4
rpl22-rps19_p4	GAACGTCCGCGAATTTGATC	TAAATTTCCGAGGACACGCA	50.0	45.0	57.9	57.5	249-278 bp	1.0	81.0
rpl22-rps19_p5	TTCTTCATAGGAACGTCCGC	ATTTCCGAGGACACGCAAAA	50.0	45.0	57.7	58.4	256-285 bp	1.0	79.7