Hotspot

trnD-GUC-trnY-GUA

LSC · 30344-30450 · 107 bp

Back to Scleromitrion

Pi 0.0374
MarkerSeek score 74.00
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
107
variable_sites
4
pi
0.0374
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 177 bp 1.0 76.1
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 176 bp 1.0 76.1
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 198 bp 1.0 75.0
trnD-GUC-trnY-GUA_p4 GTCTTTTTATTGGAGCCGCC TGGCAATATGTCTACGCTGG 50.0 50.0 57.4 57.8 240 bp 1.0 74.9
trnD-GUC-trnY-GUA_p5 GTCTTTTTATTGGAGCCGCC GGCAATATGTCTACGCTGGT 50.0 50.0 57.4 57.8 239 bp 1.0 74.9