Hotspot

petN-psbM

LSC · 28809-29496 · 688 bp

Back to Scleromitrion

Pi 0.0174

MarkerSeek score 68.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 12
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACGGCCT	50.0	47.6	58.2	58.0	833-834 bp	1.0	71.7
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TCTAGTTCCTACGGCCTTTCT	50.0	47.6	58.2	57.9	829-830 bp	1.0	71.2
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCCTTTCTACT	50.0	47.6	58.2	58.2	826-827 bp	1.0	70.5
petN-psbM_p4	TAGTGTGGGGAAGAAGTGGA	TCATTCTAGTTCCTACGGCCT	50.0	47.6	57.6	58.0	776-777 bp	1.0	69.7
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	GTTCCTACGGCCTTTCTACT	50.0	50.0	58.2	56.7	825-826 bp	1.0	69.2