Hotspot

rpoB-trnC-GCA

LSC · 26768-28020 · 1253 bp

Back to Scleromitrion

Pi 0.0149

MarkerSeek score 56.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 18
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCGCCTACACAAGCAAATTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1975-2019 bp	1.0	82.0
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATCCCCT	AAGTCTCAACAGCCCGTTTT	50.0	45.0	58.0	57.9	1383 bp	0.5	59.6
rpoB-trnC-GCA_p3	CCGCCTACACAAGCAAATTG	AAGTCTCAACAGCCCGTTTT	50.0	45.0	58.0	57.9	2062 bp	0.5	59.4
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATCCCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1340 bp	0.5	59.3
rpoB-trnC-GCA_p5	TTCCCTCATTTCCATCCCCT	GTTGAAGAGCATCCCGAAGT	50.0	50.0	58.0	57.9	1489 bp	0.5	59.2