Hotspot

trnS-GCU-trnG-GCC

LSC · 8776-9482 · 707 bp

Back to Scleromitrion

Pi 0.0227

MarkerSeek score 66.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 16
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	AAACCGAAAGCAAAGACCCT	50.0	45.0	58.0	57.9	820-858 bp	1.0	81.0
trnS-GCU-trnG-GCC_p2	CAATCCGACGCTTTAGTCCA	AACCGAAAGCAAAGACCCTT	50.0	45.0	58.0	57.9	819-857 bp	1.0	81.0
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	ACCGAAAGCAAAGACCCTTT	50.0	45.0	58.0	57.9	818-856 bp	1.0	81.0
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	AACCGAAAGCAAAGACCCTT	50.0	45.0	58.0	57.9	818-856 bp	1.0	81.0
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	ACCGAAAGCAAAGACCCTTT	50.0	45.0	58.0	57.9	817-855 bp	1.0	81.0