Hotspot

rps16-trnQ-UUG

LSC · 6021-7413 · 1393 bp

Back to Scleromitrion

Pi 0.0153

MarkerSeek score 62.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1393
variable_sites: 21
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1466-1469 bp	1.0	80.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	AAAAATCGAAATGGGGCGTG	45.0	45.0	58.1	57.6	1525-1528 bp	1.0	77.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1470-1473 bp	1.0	75.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1496-1499 bp	1.0	75.4
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1458-1461 bp	1.0	75.1