Hotspot

ndhF

SSC · 109021-111279 · 2259 bp

Pi 0.0266

MarkerSeek score 58.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 60
pi: 0.0266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATCCGCCGAAGAAGACAAAG	AGCTCATAGTGCCGTGTCTA	50.0	50.0	58.3	58.2	2765 bp	0.5	55.9
ndhF_p2	CCGCCGAAGAAGACAAAGAT	AGCTCATAGTGCCGTGTCTA	50.0	50.0	58.3	58.2	2763 bp	0.5	55.9
ndhF_p3	GATCCGCCGAAGAAGACAAA	AGCTCATAGTGCCGTGTCTA	50.0	50.0	58.3	58.2	2766 bp	0.5	55.9
ndhF_p4	ATCCGCCGAAGAAGACAAAG	TGCTCAAAATGGGTGGTTCT	50.0	45.0	58.3	57.6	2993 bp	0.5	54.8
ndhF_p5	CCGCCGAAGAAGACAAAGAT	TGCTCAAAATGGGTGGTTCT	50.0	45.0	58.3	57.6	2991 bp	0.5	54.8