Pi
0.0647
MarkerSeek score
68.20
Primer available
yes
Label
infA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| infA_p1 | TAGGGTGTCCGTCTTCTTGT | ACGGTTCGAATAGCATCCAC | 50.0 | 50.0 | 58.0 | 58.1 | 1203-1243 bp | 1.0 | 81.9 |
| infA_p2 | TAGGGTGTCCGTCTTCTTGT | ATTTTGGGCGGAATGGGAAT | 50.0 | 45.0 | 58.0 | 58.1 | 981-1021 bp | 1.0 | 81.9 |
| infA_p3 | TAGGGTGTCCGTCTTCTTGT | CTTTGCGCCATAGAAGGACT | 50.0 | 50.0 | 58.0 | 58.0 | 1085 bp | 0.5 | 59.7 |
| infA_p4 | ATAATTCGTCCGCGTCTACG | CTTTGCGCCATAGAAGGACT | 50.0 | 50.0 | 58.0 | 58.0 | 711 bp | 0.5 | 59.5 |
| infA_p5 | ATAATTCGTCCGCGTCTACG | ACGGTTCGAATAGCATCCAC | 50.0 | 50.0 | 58.0 | 58.1 | 829 bp | 0.5 | 59.3 |