Hotspot

ndhF-rpl32

SSC · 111280-111982 · 703 bp

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Pi 0.0431
MarkerSeek score 74.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
703
variable_sites
30
pi
0.0431
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTAGCTGTCGGAATAAGCA CCCTTTTTCTTCCAAATATTTCTACG 45.0 34.6 56.7 57.1 873-1155 bp 1.0 42.9
ndhF-rpl32_p2 TGTAGCTGTCGGAATAAGCAA CCCTTTTTCTTCCAAATATTTCTACG 42.9 34.6 57.4 57.1 873-1155 bp 1.0 42.9
ndhF-rpl32_p3 TTGTAGCTGTCGGAATAAGCA CCCTTTTTCTTCCAAATATTTCTACG 42.9 34.6 57.4 57.1 874-1156 bp 1.0 42.9
ndhF-rpl32_p4 TGTAGCTGTCGGAATAAGCA CCCTTTTTCTTCCAAATATTTCTACGA 45.0 33.3 56.7 58.3 873-1155 bp 1.0 42.9
ndhF-rpl32_p5 GCTGTCGGAATAAGCAAAAGT CCCTTTTTCTTCCAAATATTTCTACG 42.9 34.6 57.2 57.1 869-1151 bp 1.0 42.9