Hotspot

rps18-rpl20

LSC · 66827-67093 · 267 bp

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Pi 0.05
MarkerSeek score 77.30
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
267
variable_sites
13
pi
0.05
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 CAGTTTGAGAGAGCGGAGTC CTTGCGCAAATAGCTATAGCA 55.0 42.9 58.0 56.8 386-393 bp 1.0 66.8
rps18-rpl20_p2 CAGTTTGAGAGAGCGGAGTC ACTTGCGCAAATAGCTATAGC 55.0 42.9 58.0 56.8 387-394 bp 1.0 66.8
rps18-rpl20_p3 AGCGGAGTCGATTCCTAGAA CTTGCGCAAATAGCTATAGCA 50.0 42.9 57.9 56.8 375-382 bp 1.0 66.6
rps18-rpl20_p4 AGCGGAGTCGATTCCTAGAA ACTTGCGCAAATAGCTATAGC 50.0 42.9 57.9 56.8 376-383 bp 1.0 66.5
rps18-rpl20_p5 GCGGAGTCGATTCCTAGAAC CTTGCGCAAATAGCTATAGCA 55.0 42.9 57.9 56.8 374-381 bp 1.0 66.0