Hotspot

petG-trnW-CCA

LSC · 64720-64834 · 115 bp

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Pi 0.0609
MarkerSeek score 74.60
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
115
variable_sites
7
pi
0.0609
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 CAATACAGACGCGGTGATCA CAAAACCCGATGTCGTAGGT 50.0 50.0 58.1 57.9 187 bp 1.0 81.7
petG-trnW-CCA_p2 ACAATACAGACGCGGTGATC CAAAACCCGATGTCGTAGGT 50.0 50.0 58.1 57.9 188 bp 1.0 81.6
petG-trnW-CCA_p3 CGGTGATCAGTTGGACCTTT CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 176 bp 1.0 81.0
petG-trnW-CCA_p4 AATACAGACGCGGTGATCAG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 186 bp 1.0 80.6
petG-trnW-CCA_p5 ACAATACAGACGCGGTGATC CGCTCTTAGTTCAGTTCGGT 50.0 50.0 58.1 57.7 222 bp 1.0 79.1