Hotspot

trnE-UUC-trnT-GGU

LSC · 31018-31505 · 488 bp

Back to Scilla

Pi 0.0503
MarkerSeek score 78.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
488
variable_sites
24
pi
0.0503
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGAATCCCCGTTTTCTCCTT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 595-600 bp 1.0 83.8
trnE-UUC-trnT-GGU_p2 CGAATCCCCGTTTTCTCCTT CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 574-579 bp 1.0 83.5
trnE-UUC-trnT-GGU_p3 CGAATCCCCGTTTTCTCCTT TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 571-576 bp 1.0 83.3
trnE-UUC-trnT-GGU_p4 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 624-629 bp 1.0 83.1
trnE-UUC-trnT-GGU_p5 GGAAGTCGAATCCCCGTTTT GAACCGATGACTTACGCCTT 50.0 50.0 58.2 58.0 601-606 bp 1.0 83.1