Hotspot

psbK-psbI

LSC · 7330-7735 · 406 bp

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Pi 0.0519
MarkerSeek score 71.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
406
variable_sites
21
pi
0.0519
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACAAAGAGTTTGAGAGTAAGCA 50.0 36.4 57.9 55.6 459-465 bp 1.0 48.2
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 456-462 bp 1.0 46.2
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 457-463 bp 1.0 46.2
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT ACAAAGAGTTTGAGAGTAAGCA 45.0 36.4 58.3 55.6 458-464 bp 1.0 46.2
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG AACAAAGAGTTTGAGAGTAAGCA 50.0 34.8 57.9 56.3 460-466 bp 1.0 46.0