Hotspot

ycf3

LSC · 41704-43657 · 1954 bp

Back to Schweinfurthia

Pi 0

MarkerSeek score 57.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1954
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACAAAGAGACCTGCCAACAG	TCGGAATAGTTGCCTTCGTT	50.0	45.0	58.0	57.2	2915-2943 bp	1.0	73.8
ycf3_p2	CAAAGAGACCTGCCAACAGT	TCGGAATAGTTGCCTTCGTT	50.0	45.0	58.0	57.2	2914-2942 bp	1.0	73.8
ycf3_p3	AATTAAAAATCACGCGCGCA	TCGGAATAGTTGCCTTCGTT	40.0	45.0	58.0	57.2	2626-2654 bp	1.0	73.8
ycf3_p4	TTAAAAATCACGCGCGCATT	TCGGAATAGTTGCCTTCGTT	40.0	45.0	58.0	57.2	2624-2652 bp	1.0	73.8
ycf3_p5	GAGACCTGCCAACAGTCAAA	TCGGAATAGTTGCCTTCGTT	50.0	45.0	58.0	57.2	2910-2938 bp	1.0	73.7