Hotspot

ndhD

SSC · 114832-116361 · 1530 bp

Back to Schweinfurthia

Pi 0.000654

MarkerSeek score 59.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 1
pi: 0.000654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACGTAGGAAAAGAAGAAAAAGCC	TTACCGACAAAATCCGTGCT	39.1	45.0	57.6	57.8	1681 bp	1.0	51.2
ndhD_p2	ACGTAGGAAAAGAAGAAAAAGCC	TACCGACAAAATCCGTGCTC	39.1	50.0	57.6	58.3	1680 bp	1.0	50.3
ndhD_p3	CGTAGGAAAAGAAGAAAAAGCCT	TTACCGACAAAATCCGTGCT	39.1	45.0	57.3	57.8	1680 bp	1.0	49.1
ndhD_p4	CGTAGGAAAAGAAGAAAAAGCCT	TACCGACAAAATCCGTGCTC	39.1	50.0	57.3	58.3	1679 bp	1.0	48.3
ndhD_p5	ACGTAGGAAAAGAAGAAAAAGCC	CGACAAAATCCGTGCTCAAA	39.1	45.0	57.6	57.3	1677 bp	1.0	47.1