Hotspot

rpl22-rps19

LSC · 83377-83438 · 62 bp

Back to Schweinfurthia

Pi 0

MarkerSeek score 40.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	AATTTCTTCGCCGTAGTCCC	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	895-896 bp	1.0	79.4
rpl22-rps19_p2	CGCATAACTACGTCCTCGAG	AAAAGTTTGGGTAGAGCCGG	55.0	50.0	58.0	58.1	926-927 bp	1.0	79.4
rpl22-rps19_p3	AATTTCTTCGCCGTAGTCCC	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.0	58.1	977-978 bp	1.0	79.3
rpl22-rps19_p4	CGCATAACTACGTCCTCGAG	ATTACCTTCTGGGGAGGTCC	55.0	55.0	58.0	58.1	1008-1009 bp	1.0	79.3
rpl22-rps19_p5	AATTTCTTCGCCGTAGTCCC	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.0	58.1	968-969 bp	1.0	79.3