Hotspot

petA-psbJ

LSC · 62749-63771 · 1023 bp

Back to Schweinfurthia

Pi 0.000978

MarkerSeek score 59.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 1
pi: 0.000978
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	1132 bp	1.0	76.2
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.0	57.4	1131 bp	1.0	75.7
petA-psbJ_p3	TGGCATCTGTGATTTTGGCA	TCCGGATTAGGTTCATCCCT	45.0	50.0	58.4	57.5	1133 bp	1.0	73.2
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1159 bp	1.0	72.8
petA-psbJ_p5	TGGCATCTGTGATTTTGGCA	CCGGATTAGGTTCATCCCTG	45.0	55.0	58.4	57.4	1132 bp	1.0	72.7