Hotspot

psbI-trnS-GCU

LSC · 7912-8033 · 122 bp

Back to Schweinfurthia

Pi 0
MarkerSeek score 53.30
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
122
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 ATCTAATGATCCGGGGCGTA TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 234-235 bp 1.0 79.8
psbI-trnS-GCU_p2 TATCTAATGATCCGGGGCGT TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 235-236 bp 1.0 79.8
psbI-trnS-GCU_p3 ATCTAATGATCCGGGGCGTA GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 235-236 bp 1.0 79.8
psbI-trnS-GCU_p4 TATCTAATGATCCGGGGCGT GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 236-237 bp 1.0 79.8
psbI-trnS-GCU_p5 ATCTAATGATCCGGGGCGTA ATTGGGAGAGATGGCTGAGT 50.0 50.0 58.1 58.1 253-254 bp 1.0 78.9