Hotspot

ndhF

SSC · 112243-114459 · 2217 bp

Back to Schrebera

Pi 0.00908

MarkerSeek score 50.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 44
pi: 0.00908
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGCCGAAGAAGATCCTTCTC	TAACGCTGTCCCATATCCCT	55.0	50.0	58.2	57.9	2996-3000 bp	0.5	58.8
ndhF_p2	GCCGAAGAAGATCCTTCTCC	TAACGCTGTCCCATATCCCT	55.0	50.0	57.8	57.9	2995-2999 bp	0.5	58.2
ndhF_p3	CGCCGAAGAAGATCCTTCTC	GCTGTCCCATATCCCTTCCT	55.0	55.0	58.2	58.6	2992-2996 bp	0.5	55.1
ndhF_p4	GCCGAAGAAGATCCTTCTCC	GCTGTCCCATATCCCTTCCT	55.0	55.0	57.8	58.6	2991-2995 bp	0.5	54.5
ndhF_p5	GAGATCCGCCGAAGAAGATC	GCTGTCCCATATCCCTTCCT	55.0	55.0	57.9	58.6	2998-3000 bp	0.333333	49.1