Hotspot

atpB

LSC · 55059-56555 · 1497 bp

Back to Schrebera

Pi 0.00312

MarkerSeek score 38.70

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 11
pi: 0.00312
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	TCGATTAGGGGTCAGTACACA	AACTGATCTCCGTGCTATCG	47.6	50.0	57.9	57.2	1612 bp	1.0	65.6
atpB_p2	CGATTAGGGGTCAGTACACA	AACTGATCTCCGTGCTATCG	50.0	50.0	56.4	57.2	1611 bp	1.0	61.2
atpB_p3	CGATTAGGGGTCAGTACACAA	AACTGATCTCCGTGCTATCG	47.6	50.0	57.1	57.2	1611 bp	1.0	59.2
atpB_p4	TCGATTAGGGGTCAGTACAC	AACTGATCTCCGTGCTATCG	50.0	50.0	56.1	57.2	1612 bp	1.0	58.9
atpB_p5	CCCAAACAATTCGATTAGGGG	AACTGATCTCCGTGCTATCG	47.6	50.0	56.9	57.2	1622 bp	1.0	57.7