Hotspot

rpl32-trnL(UAG)

SSC · 115095-116007 · 913 bp

Back to Schrebera

Pi 0.013

MarkerSeek score 47.90

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 913
variable_sites: 23
pi: 0.013
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	GTTGCCGTCGGAAAAAGAAG	AAAAATAGCACTTGGGCCGA	50.0	45.0	58.0	58.1	1769-2140 bp	1.0	85.1
rpl32-trnL(UAG)_p2	GTTGCCGTCGGAAAAAGAAG	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1789-2160 bp	1.0	85.0
rpl32-trnL(UAG)_p3	GTTGCCGTCGGAAAAAGAAG	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	1790-2161 bp	1.0	85.0
rpl32-trnL(UAG)_p4	TGCCGTCGGAAAAAGAAGAA	AAAAATAGCACTTGGGCCGA	45.0	45.0	58.0	58.1	1767-2138 bp	1.0	85.0
rpl32-trnL(UAG)_p5	TTGCCGTCGGAAAAAGAAGA	AAAAATAGCACTTGGGCCGA	45.0	45.0	58.0	58.1	1768-2139 bp	1.0	85.0