Hotspot

rpl22-rps19

LSC · 86177-86241 · 65 bp

Back to Schrebera

Pi 0.0185

MarkerSeek score 54.30

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 3
pi: 0.0185
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TAGGAGCGTCCACGAATTTG	GCGATAATAGATCTCGGCGT	50.0	50.0	58.0	57.5	191-197 bp	1.0	79.7
rpl22-rps19_p2	AGCGTCCACGAATTTGATCA	GCGATAATAGATCTCGGCGT	45.0	50.0	57.9	57.5	187-193 bp	1.0	79.1
rpl22-rps19_p3	TAGGAGCGTCCACGAATTTG	AGCGATAATAGATCTCGGCG	50.0	50.0	58.0	57.2	192-198 bp	1.0	77.4
rpl22-rps19_p4	AGCGTCCACGAATTTGATCA	AGCGATAATAGATCTCGGCG	45.0	50.0	57.9	57.2	188-194 bp	1.0	76.8
rpl22-rps19_p5	TAGGAGCGTCCACGAATTTG	TCCGAGGACATGCAAAAAGC	50.0	50.0	58.0	59.1	209-215 bp	1.0	74.8