Hotspot

rps12-clpP

LSC · 72394-72526 · 133 bp

Back to Schrebera

Pi 0.017

MarkerSeek score 52.40

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 5
pi: 0.017
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CGGGGGATTTCGTGACATTT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.5	57.9	258-274 bp	1.0	76.3
rps12-clpP_p2	GTACATGTTCCTCGACGCTG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.7	57.9	295-311 bp	1.0	74.6
rps12-clpP_p3	GGGGATTTCGTGACATTTCG	TATGTCAGCAACAGAAGCCC	50.0	50.0	57.2	57.9	256-272 bp	1.0	74.0
rps12-clpP_p4	AGTACATGTTCCTCGACGCT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.8	57.9	296-312 bp	1.0	73.8
rps12-clpP_p5	TACATGTTCCTCGACGCTGA	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.8	57.9	294-310 bp	1.0	73.8