Hotspot

trnG(GCC)-tRNA-fM(CAU)

LSC · 38277-38473 · 197 bp

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Pi 0.0139
MarkerSeek score 50.20
Primer available yes
Label trnG(GCC)-tRNA-fM(CAU)

Feature Metadata

label_name
trnG(GCC)-tRNA-fM(CAU)
feature_type
igs
region
LSC
length_bp
197
variable_sites
7
pi
0.0139
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG(GCC)-tRNA-fM(CAU)_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 250-266 bp 1.0 85.4
trnG(GCC)-tRNA-fM(CAU)_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 251-267 bp 1.0 85.4
trnG(GCC)-tRNA-fM(CAU)_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 286-302 bp 1.0 85.2
trnG(GCC)-tRNA-fM(CAU)_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 287-303 bp 1.0 85.2
trnG(GCC)-tRNA-fM(CAU)_p5 AGAAGATGCGGGTTCGATTC TCACTTCTTCTCTTCAGCGC 50.0 50.0 58.1 57.9 307-323 bp 1.0 84.7