Hotspot

trnM(CAU)-psbD

LSC · 33289-34619 · 1331 bp

Back to Schrebera

Pi 0.00742
MarkerSeek score 61.40
Primer available yes
Label trnM(CAU)-psbD

Feature Metadata

label_name
trnM(CAU)-psbD
feature_type
igs
region
LSC
length_bp
1331
variable_sites
23
pi
0.00742
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM(CAU)-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1474-1491 bp 1.0 81.1
trnM(CAU)-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1477-1494 bp 1.0 80.9
trnM(CAU)-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1464-1481 bp 1.0 80.1
trnM(CAU)-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1467-1484 bp 1.0 79.9
trnM(CAU)-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1451-1468 bp 1.0 79.4