Hotspot

trnH(GUG)-psbA

LSC · 82-525 · 444 bp

Back to Schrebera

Pi 0.0178
MarkerSeek score 46.60
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
444
variable_sites
18
pi
0.0178
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 ACTGCCTTAATCCACTTGGC GAGCTTAGTTTCCGTCTGGG 50.0 55.0 58.2 58.0 1131-1142 bp 1.0 82.5
trnH(GUG)-psbA_p2 CTGCCTTAATCCACTTGGCT GAGCTTAGTTTCCGTCTGGG 50.0 55.0 57.9 58.0 1130-1141 bp 0.666667 69.0
trnH(GUG)-psbA_p3 CTGCCTTAATCCACTTGGCT GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1136-1147 bp 0.666667 69.0
trnH(GUG)-psbA_p4 CTGCCTTAATCCACTTGGCT GGAGCTTAGTTTCCGTCTGG 50.0 55.0 57.9 58.0 1131-1142 bp 0.666667 69.0
trnH(GUG)-psbA_p5 CTGCCTTAATCCACTTGGCT GGGAGCTTAGTTTCCGTCTG 50.0 55.0 57.9 58.0 1132-1143 bp 0.666667 69.0