Hotspot

petD

LSC · 79652-80876 · 1225 bp

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Pi 0.00163

MarkerSeek score 59.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1225
variable_sites: 2
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGTTTCCCCTCAACTTCACA	CGTTTCAAAGCGAATTCCCC	45.0	50.0	56.9	58.0	1388 bp	1.0	71.0
petD_p2	GGATGTTTCCCCTCAACTTCA	CGTTTCAAAGCGAATTCCCC	47.6	50.0	57.8	58.0	1391 bp	1.0	70.7
petD_p3	TGGATGTTTCCCCTCAACTTC	CGTTTCAAAGCGAATTCCCC	47.6	50.0	57.8	58.0	1392 bp	1.0	70.7
petD_p4	TGGATGTTTCCCCTCAACTT	CGTTTCAAAGCGAATTCCCC	45.0	50.0	56.6	58.0	1392 bp	1.0	69.0
petD_p5	TTGGATGTTTCCCCTCAACT	CGTTTCAAAGCGAATTCCCC	45.0	50.0	56.6	58.0	1393 bp	1.0	69.0