Hotspot

ndhF-rpl32

SSC · 115945-116943 · 999 bp

Back to Schotia

Pi 0.00206
MarkerSeek score 62.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
999
variable_sites
2
pi
0.00206
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGTCGGAAAAAGGAGAAGT AGAAAGAGATGTGGCTAACGA 50.0 42.9 57.8 56.8 1183-1210 bp 1.0 62.8
ndhF-rpl32_p2 TTTGTTGCCGTCGGAAAAAG AGAAAGAGATGTGGCTAACGA 45.0 42.9 57.8 56.8 1190-1217 bp 1.0 62.5
ndhF-rpl32_p3 CACACACGGCGAAGATTTTT AGAAAGAGATGTGGCTAACGA 45.0 42.9 57.6 56.8 1208-1235 bp 1.0 60.8
ndhF-rpl32_p4 CCGTCGGAAAAAGGAGAAGT AGAAAGAGATGTGGCTAACGAA 50.0 40.9 57.8 57.5 1183-1210 bp 1.0 60.4
ndhF-rpl32_p5 TTTGTTGCCGTCGGAAAAAG AGAAAGAGATGTGGCTAACGAA 45.0 40.9 57.8 57.5 1190-1217 bp 1.0 60.1