Hotspot

accD-psaI

LSC · 62328-62909 · 582 bp

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Pi 0.00178

MarkerSeek score 62.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 1
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTTTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	705-725 bp	1.0	81.7
accD-psaI_p2	AAGGGCGTTTTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	704-724 bp	1.0	81.7
accD-psaI_p3	TCAATCGTACCGCGTAATCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	728-748 bp	1.0	81.1
accD-psaI_p4	CAATCGTACCGCGTAATCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	727-747 bp	1.0	81.0
accD-psaI_p5	AAGGGCGTTTTGAGTGAGTT	AACGAAGCCATTGCAATTGC	45.0	45.0	57.9	58.6	711-731 bp	1.0	77.4