Hotspot

rbcL-accD

LSC · 59930-60833 · 904 bp

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Pi 0.00332

MarkerSeek score 60.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 904
variable_sites: 3
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TTAGTTAGCCCACACCCGTA	50.0	50.0	58.0	58.1	1047 bp	1.0	79.4
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TTAGTTAGCCCACACCCGTA	55.0	50.0	58.0	58.1	1062 bp	1.0	79.4
rbcL-accD_p3	AGTCCTGAATTAGCTGCTGC	TAGTTAGCCCACACCCGTAT	50.0	50.0	58.0	57.8	1046 bp	1.0	78.6
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	TAGTTAGCCCACACCCGTAT	55.0	50.0	58.0	57.8	1061 bp	1.0	78.6
rbcL-accD_p5	AGTCCTGAATTAGCTGCTGC	GCCCACACCCGTATTCTAAC	50.0	55.0	58.0	58.3	1040 bp	1.0	77.2