Hotspot

trnT-UGU-trnL-UAA

LSC · 49533-50444 · 912 bp

Back to Schotia

Pi 0.00225

MarkerSeek score 62.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 2
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	949-970 bp	1.0	80.4
trnT-UGU-trnL-UAA_p2	TAGGGGAAAAGAAAAGCCGG	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1006-1027 bp	1.0	79.7
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	981-1002 bp	1.0	79.5
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	955-976 bp	1.0	79.0
trnT-UGU-trnL-UAA_p5	TAGGGGAAAAGAAAAGCCGG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1012-1033 bp	1.0	78.3