Hotspot

trnR-UCU-atpA

LSC · 10295-10742 · 448 bp

Back to Schotia

Pi 0.00703

MarkerSeek score 52.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 3
pi: 0.00703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTTACCGGGGAAGCAGAA	47.6	50.0	57.3	57.9	543-562 bp	1.0	69.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTTACCGGGGAAGC	47.6	50.0	57.3	57.9	547-566 bp	1.0	68.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	533-552 bp	1.0	67.0
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTTACCGGGGAAGCAGAAG	47.6	50.0	57.3	57.6	542-561 bp	1.0	66.6
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CCAAGACATTTACCGGGGAA	47.6	50.0	57.3	57.5	549-568 bp	1.0	65.9