Hotspot

rpl32-trnL-UAG

Genome · 100187-100679 · 493 bp

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Pi 0.0205
MarkerSeek score 58.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
493
variable_sites
10
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCCAAATCTCTTTCTACTCGA AGCGTGTCTACCGATTTCAC 40.9 50.0 56.9 58.0 574-583 bp 1.0 57.4
rpl32-trnL-UAG_p2 AGCCAAATCTCTTTCTACTCGA CAGCGTGTCTACCGATTTCA 40.9 50.0 56.9 58.0 575-584 bp 1.0 57.4
rpl32-trnL-UAG_p3 AGCCAAATCTCTTTCTACTCGA TCCTAAGAGCAGCGTGTCTA 40.9 50.0 56.9 57.9 584-593 bp 1.0 56.4
rpl32-trnL-UAG_p4 AGCCAAATCTCTTTCTACTCGA CCTAAGAGCAGCGTGTCTAC 40.9 55.0 56.9 57.8 583-592 bp 1.0 55.9
rpl32-trnL-UAG_p5 AGCCAAATCTCTTTCTACTCGA CTAAGAGCAGCGTGTCTACC 40.9 55.0 56.9 57.8 582-591 bp 1.0 55.9