Hotspot

trnR-ACG-trnN-GUU

Genome · 98779-99212 · 434 bp

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Pi 0.00483

MarkerSeek score 62.50

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 434
variable_sites: 2
pi: 0.00483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCCTGTTGTTCCTCAGTAGC	55.0	55.0	57.8	57.9	563-577 bp	1.0	80.6
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	602-616 bp	1.0	80.4
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	525-539 bp	1.0	80.4
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	512-526 bp	1.0	80.4
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	639-653 bp	1.0	79.7