Hotspot

atpB-rbcL

Genome · 50649-51380 · 732 bp

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Pi 0.00552

MarkerSeek score 62.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 732
variable_sites: 4
pi: 0.00552
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	878-890 bp	1.0	72.5
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	877-889 bp	1.0	72.2
atpB-rbcL_p3	GGAAACCCCAAGACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	820-832 bp	1.0	72.2
atpB-rbcL_p4	GGAAACCCCAAGACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	819-831 bp	1.0	72.0
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	876-888 bp	1.0	71.9