Hotspot

trnV-UAC-trnM-CAU

Genome · 48344-48486 · 143 bp

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Pi 0.042

MarkerSeek score 74.80

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: Genome
length_bp: 143
variable_sites: 6
pi: 0.042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	CCTAACGGAGCTATAGCCCT	CGCCGTATGAAAGCAGTACT	55.0	50.0	58.1	58.1	205-223 bp	1.0	81.0
trnV-UAC-trnM-CAU_p2	ACCTAACGGAGCTATAGCCC	CGCCGTATGAAAGCAGTACT	55.0	50.0	58.4	58.1	206-224 bp	1.0	78.7
trnV-UAC-trnM-CAU_p3	GATGGACTCCCCCGAAAAAG	CGCCGTATGAAAGCAGTACT	55.0	50.0	58.2	58.1	255 bp	0.5	57.4
trnV-UAC-trnM-CAU_p4	ATCTCATGATGGACTCCCCC	CGCCGTATGAAAGCAGTACT	55.0	50.0	58.3	58.1	262 bp	0.5	57.3
trnV-UAC-trnM-CAU_p5	GGGTAAACGAGGTGCTCTAC	CGCCGTATGAAAGCAGTACT	55.0	50.0	57.7	58.1	224 bp	0.5	57.1