Hotspot

trnS-GCU-trnG-UCC

Genome · 7837-8280 · 444 bp

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Pi 0.018

MarkerSeek score 56.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: Genome
length_bp: 444
variable_sites: 8
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCACCGCTTTAGTCCA	GAACGAATCGCGCTTTTACC	50.0	50.0	57.9	58.2	518-525 bp	1.0	78.7
trnS-GCU-trnG-UCC_p2	AATCCACCGCTTTAGTCCAC	GAACGAATCGCGCTTTTACC	50.0	50.0	57.9	58.2	517-524 bp	1.0	78.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GAACGAATCGCGCTTTTACC	50.0	50.0	58.2	58.2	572-579 bp	1.0	78.3
trnS-GCU-trnG-UCC_p4	AGCAATCCACCGCTTTAGTC	GAACGAATCGCGCTTTTACC	50.0	50.0	58.3	58.2	520-527 bp	1.0	77.5
trnS-GCU-trnG-UCC_p5	CACCGCTTTAGTCCACTCAG	GAACGAATCGCGCTTTTACC	55.0	50.0	58.3	58.2	513-520 bp	1.0	77.3