Hotspot

psbI-trnS-GCU

Genome · 7648-7748 · 101 bp

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Pi 0.0297

MarkerSeek score 64.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 101
variable_sites: 3
pi: 0.0297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCCTGTCTAACGATCCAGGG	TGGACTAAAGCGGTGGATTG	55.0	50.0	58.2	57.9	218-226 bp	1.0	78.4
psbI-trnS-GCU_p2	TCCTGTCTAACGATCCAGGG	GTGGACTAAAGCGGTGGATT	55.0	50.0	58.2	57.9	219-227 bp	1.0	78.4
psbI-trnS-GCU_p3	TCTAACGATCCAGGGCGTAA	TGGACTAAAGCGGTGGATTG	50.0	50.0	58.2	57.9	213-221 bp	1.0	78.4
psbI-trnS-GCU_p4	TCTAACGATCCAGGGCGTAA	GTGGACTAAAGCGGTGGATT	50.0	50.0	58.2	57.9	214-222 bp	1.0	78.4
psbI-trnS-GCU_p5	TCCTGTCTAACGATCCAGGG	ATTGGGAGAGATGGCTGAGT	55.0	50.0	58.2	58.1	237-245 bp	1.0	78.3