Hotspot

ycf1

SSC · 114502-119829 · 5328 bp

Back to Schoenorchis

Pi 0.0289

MarkerSeek score 56.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 152
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAATGGATTTTTCAAACCCCC	TCCAAAGGAAATTGCTATATCT	42.9	31.8	55.9	52.9	263 bp	1.0	43.7
ycf1_p2	CAATGGATTTTTCAAACCCCC	TCCAAAGGAAATTGCTATATCTT	42.9	30.4	55.9	53.8	263 bp	1.0	43.7
ycf1_p3	CAATGGATTTTTCAAACCCCC	ATCCAAAGGAAATTGCTATATCT	42.9	30.4	55.9	53.5	264 bp	1.0	43.6
ycf1_p4	CAATGGATTTTTCAAACCCCC	AATCCAAAGGAAATTGCTATATCT	42.9	29.2	55.9	54.3	265 bp	1.0	43.6
ycf1_p5	CAATGGATTTTTCAAACCCCC	ATCCAAAGGAAATTGCTATATCTT	42.9	29.2	55.9	54.3	264 bp	1.0	43.6