Hotspot

rps7-trnL-CAA

IRa · 131440-132167 · 728 bp

Back to Schoenorchis

Pi 0.0289

MarkerSeek score 75.60

Primer available yes

Label rps7-trnL-CAA

Feature Metadata

label_name: rps7-trnL-CAA
feature_type: igs
region: IRa
length_bp: 728
variable_sites: 21
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-trnL-CAA_p1	ATGGCAGAGGCAAATAGAGC	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.0	58.0	799-2549 bp	1.0	80.9
rps7-trnL-CAA_p2	ATGGCAGAGGCAAATAGAGC	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.0	58.0	797-2547 bp	1.0	80.9
rps7-trnL-CAA_p3	GGCAGAGGCAAATAGAGCTT	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.0	58.0	797-2547 bp	1.0	80.6
rps7-trnL-CAA_p4	GGCAGAGGCAAATAGAGCTT	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.0	58.0	795-2545 bp	1.0	80.6
rps7-trnL-CAA_p5	ATGGCAGAGGCAAATAGAGC	GCGTGTCTACCATTTCACCA	50.0	50.0	58.0	58.2	794-2544 bp	1.0	79.4