Hotspot

trnN-GUU-rpl32

SSC · 107806-108484 · 679 bp

Back to Schoenorchis

Pi 0.0421

MarkerSeek score 73.00

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 679
variable_sites: 27
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AAAGCGAGCCCTTTCATTCA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	931-1207 bp	1.0	84.4
trnN-GUU-rpl32_p2	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	930-1206 bp	1.0	84.4
trnN-GUU-rpl32_p3	TCAAAGCGAGCCCTTTCATT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	933-1209 bp	1.0	84.4
trnN-GUU-rpl32_p4	TATGCCGAGGTATTGAGGGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	57.9	58.0	1043-1319 bp	1.0	83.8
trnN-GUU-rpl32_p5	GGCAGAATAGCAGAGCAAGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	1213-1489 bp	1.0	83.7