Hotspot

trnL-CAA-rps7

IRb · 95773-96500 · 728 bp

Back to Schoenorchis

Pi 0.0289
MarkerSeek score 75.60
Primer available yes
Label trnL-CAA-rps7

Feature Metadata

label_name
trnL-CAA-rps7
feature_type
igs
region
IRb
length_bp
728
variable_sites
21
pi
0.0289
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-rps7_p1 GTCTCGCGTGTCTACCATTT ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 799-2549 bp 1.0 80.9
trnL-CAA-rps7_p2 CTCGCGTGTCTACCATTTCA ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.0 58.0 797-2547 bp 1.0 80.9
trnL-CAA-rps7_p3 GTCTCGCGTGTCTACCATTT GGCAGAGGCAAATAGAGCTT 50.0 50.0 58.0 58.0 797-2547 bp 1.0 80.6
trnL-CAA-rps7_p4 CTCGCGTGTCTACCATTTCA GGCAGAGGCAAATAGAGCTT 50.0 50.0 58.0 58.0 795-2545 bp 1.0 80.6
trnL-CAA-rps7_p5 GCGTGTCTACCATTTCACCA ATGGCAGAGGCAAATAGAGC 50.0 50.0 58.2 58.0 794-2544 bp 1.0 79.4