Hotspot

petD-rpoA

LSC · 78498-78677 · 180 bp

Back to Schoenorchis

Pi 0.0615

MarkerSeek score 74.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 11
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTTTTGATCGGTACCGCAGT	TTGAGCACTGTACAAAAGCA	45.0	40.0	57.8	55.8	329-330 bp	1.0	64.0
petD-rpoA_p2	GTCCAGTAGCCACAACAGTC	TTGAGCACTGTACAAAAGCA	55.0	40.0	58.2	55.8	349-350 bp	1.0	63.6
petD-rpoA_p3	TCCAGTAGCCACAACAGTCT	TTGAGCACTGTACAAAAGCA	50.0	40.0	58.3	55.8	348-349 bp	1.0	63.0
petD-rpoA_p4	GGTACCGCAGTAGCTCTTTG	TTGAGCACTGTACAAAAGCA	55.0	40.0	58.4	55.8	320-321 bp	1.0	62.6
petD-rpoA_p5	GTACCGCAGTAGCTCTTTGG	TTGAGCACTGTACAAAAGCA	55.0	40.0	58.4	55.8	319-320 bp	1.0	62.6