Hotspot

ndhC-trnV-UAC

LSC · 50378-51029 · 652 bp

Back to Schoenorchis

Pi 0.0787

MarkerSeek score 83.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 652
variable_sites: 45
pi: 0.0787
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGGAATGCCCAGAAAATCTCA	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.3	57.8	700-708 bp	1.0	73.6
ndhC-trnV-UAC_p2	AGGAATGCCCAGAAAATCTCA	AGCCCAATGCAACAAAATGC	42.9	45.0	57.3	58.5	782-790 bp	1.0	70.8
ndhC-trnV-UAC_p3	AGGAATGCCCAGAAAATCTCA	GAAGGTCTACGGTTCGAGTC	42.9	55.0	57.3	57.5	699-707 bp	1.0	70.8
ndhC-trnV-UAC_p4	AGGAATGCCCAGAAAATCTCA	GTTCGAGTCCGTATAGCCCT	42.9	55.0	57.3	58.7	688-696 bp	1.0	69.8
ndhC-trnV-UAC_p5	AGGAATGCCCAGAAAATCTCAT	AGAAGGTCTACGGTTCGAGT	40.9	50.0	57.7	57.8	700-708 bp	1.0	69.4